Terminator

Part:BBa_K187025:Design

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-18)


terminator in pAB, BioBytes plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 9
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 53
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 9
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 9
    Illegal PstI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.


Source

The terminator is derived from part b1006. pAB is entered as part K187000.

References