Terminator
Part:BBa_K187025:Design
Designed by: Julia Pon Group: iGEM09_Alberta (2009-10-18)
terminator in pAB, BioBytes plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 9
Illegal PstI site found at 53 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 53
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 9
Illegal PstI site found at 53 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 9
Illegal PstI site found at 53 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
A terminator with long sticky ends can be prepared from this plasmid and assembled downstream of a gene using the Biobytes method. The addition of one DNA segment with the BioBytes method takes only 20 minutes. This ability to easily 'mix and match' terminators and genes allows rapid testing of different terminator designs.
Source
The terminator is derived from part b1006. pAB is entered as part K187000.